Description
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human Tumor Necrosis Factor ligand Superfamily Member 10, TNFSF10 / Trail present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human Tumor Necrosis Factor ligand Superfamily Member 10, TNFSF10 / Trail in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm